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. 2010 May 27;21(7):891–901. doi: 10.1089/hum.2009.148

FIG. 5.

FIG. 5.

Anti-F1 antibodies and survival of mice immunized in a prime–boost regimen with AdLacZ-pIX/F1 or F1/Alhydrogel. BALB/c mice (n = 11 to 22 per group) were immunized with AdLacZ-pIX/F1 or purified F1/Alhydrogel. A subset of animals received a single repeat administration of the same immunogens at the same dose 4 weeks after primary immunization. (A) Serum anti-F1 titers, AdLacZ-pIX/F1 (109 particle units, pu). Six weeks after the prime immunization serum antibody levels were measured in an anti-F1-specific ELISA (p < 0.01, AdLacZ-pIX/F1 unboosted or boosted vs. AdLacZ). (B) Serum anti-F1 titers, purified recombinant F1 (5.1 ng, the molar equivalent amount of protein present in 109 pu of AdLacZ-pIX/F1) with Alhydrogel (p < 0.01, F1 unboosted or boosted vs. naive). (C) Survival, AdLacZ-pIX/F1. Six weeks after prime immunization with 109 pu of AdLacZ-pIX/F1, mice were challenged with a lethal dose of Yersinia pestis CO92, and survival of the animals was monitored for 14 days. Naive mice and mice that received AdLacZ (1011 pu) intramuscularly were included as negative controls (p < 0.0001, AdLacZ-pIX/F1 vs. AdLacZ; p < 0.0001, AdLacZ-pIX/F1 plus boost vs. AdLacZ). (D) Survival, F1/Alhydrogel. Naive mice were used as negative controls (p < 0.0007, F1 vs. naive; p = 0.0044, F1 plus boost vs. naive).