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. 2010 May 13;21(7):829–842. doi: 10.1089/hum.2009.172

FIG. 1.

FIG. 1.

Effect of papaverine and saline preinjections on luciferase expression in rat and rhesus monkey limbs. (A) Rats had either a preinjection of 1.5 ml of papaverine solution (n = 9) or saline (n = 7) followed 2 min later by a 3.0-ml hydrodynamic limb vein (HLV) injection of pDNA (250 μg of pCMV-Luc). Animals were killed 5 days later and limb muscles were analyzed for luciferase activity. Average luciferase in each group is shown. (B) One rhesus monkey had preinjections of papaverine (two limbs) or saline (two limbs) followed by HLV injections containing 5 mg of pCMV-Luc. The total injection volume (papaverine or saline and pDNA solution) was 0.35 ml/ml limb volume and the tourniquet pressure was 700 mmHg. The total luciferase in each injected limb is shown, as well as the day 7 equivalent for the day 4 samples (see Materials and Methods).