FIG. 4.

Postinjection tourniquet time versus luciferase expression. (A) Three groups of rats (n = 4) had HLV injections of 250 μg of pCMV-Luc. The tourniquet was removed at 120 sec, 20 sec, or immediately after injection. Animals were killed 7 days after injection and limb muscles were analyzed for luciferase activity. (B) Two monkeys had HLV injections in all four limbs. The pDNA concentration was 180 μg/ml and the injection volume was 0.45 ml/ml limb volume. The postinjection tourniquet time was either 20 sec (five limbs) or 120 sec (three limbs). The total luciferase in each limb is shown with the day 4 correction (see Materials and Methods).