FIG. 2.

Analysis of Artemis expression in transduced cell populations. (A) Expression of Artemis protein was verified by Western blot analysis (78 kDa) of nuclear extracts derived from tMEFSCIDA wild-type (+/+), Artemis heterozygous (+/−), Artemis-deficient (−/−), and Artemis-deficient MEFs transduced with the ABiG lentiviral vector (−/−t) or the control CSIIEG lentiviral vector (−/−c). (B) Artemis activity was detected in a fluorescence hairpin-opening assay. Nuclear extracts generated from tMEFSCIDA wild-type (+/+), Artemis heterozygous (+/−), and Artemis deficient (−/−) as well as Artemis-deficient MEFs transduced at increasing multiplicity with ABiG lentiviral vector were assayed for hairpin opening activity as described in Materials and Methods. 0, no lysate; −, not transduced. Each value represents the mean of three replicates ± SD.