FIG. 5.

The effect of promoter strength on Artemis toxicity. The effect of promoter strength on Artemis toxicity was assayed by transducing (A) tMEFSCIDA^+/+, (B) tMEFSCIDA^+/−, and (C) tMEFSCIDA^−/− cells at increasing MOI with EF1α-hArtemis, PGK-hArtemis, and the control vector EF1α-Puromycin. To control for integrant frequency, side-by-side titering experiments indicated a consistent copy number between murine NIH 3T3 and tMEFSCIDA cell lines by quantitative PCR (see Materials and Methods) for all three vectors. Cell viability was assayed 5 days later by trypan blue exclusion, presented here as the percentage of an untreated cell population. Each value represents the mean of three replicates ± SD.