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Comet assay of genomic DNA damage. The comet assay was performed on tMEFSCIDA−/− cells transduced with either CSIIEG or EAIG. Transduced cells were collected by GFP-positive cell sorting and subjected to alkaline comet assay as described in Materials and Methods. Mean tail length ± SD (n = 3) is plotted for Artemis-deficient MEFs transduced at various multiplicities with EAIG and CSIIEG lentiviral vectors.
