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Induction of apoptosis in Artemis-transduced cells. NIH 3T3 cells were transduced at increasing MOI with (A) CSIIEG or (B) EAIG lentiviral vectors and then 20 hr later stained with Annexin V and 7-AAD. Transduced GFP-positive cells were gated to determine Annexin V binding and 7-AAD staining in each population, expressed here as the singly and doubly staining cells as a percentage of the whole GFP+ cell population ± SD (n = 3).
