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. 2010 Aug 10;5(8):e12105. doi: 10.1371/journal.pone.0012105

Figure 4. Increased cell death in the thymus of the 15 week old Hexb −/− FcRγ +/+ mouse.

Figure 4

(A) Thymic sections from 15 week old Hexb −/− FcRγ+/+ and Hexb +/− FcRγ+/+ mice were analyzed by TEM. Left panel, at lower magnification many electron dense materials and vacuoles are evident. Middle panel, nucleus-like electron dense particles and vacuoles are visible in the cytoplasm of the macrophages at higher magnification. Right panel, nuclear particles were not confirmed in the thymus of the Hexb +/− FcRγ+/+ mouse.N, nucleus particle-like structure; MN, macrophage nucleus. (B) Paraffin-embedded thymic sections from Hexb +/− FcRγ+/+, Hexb −/− FcRγ+/+ and Hexb −/− FcRγ −/− mice were stained using the TUNEL method. Scale bar, 50((m. A higher magnification image of the arrow position in the middle panel is shown in the right shoulder frame. (C) Thymic cells from Hexb +/− FcR+/+, Hexb −/− FcR+/+ and Hexb −/− FcR −/− mice were stained with FITC conjugated anti-Annexin V antibody and PI, and the percentages of dying cells were determined by flow cytometry (n = 4–8). *P<0.01. (D) IgG deposition in TCR(-positive T cells analyzed by flow cytometry (n = 3–5). *P≤0.01.