Dag (cDNA) |
5′-GAGGACCAGGCCACCTTTATTAAG-3′ (forward primer)5′-TTAAGGGGGAACATACGGAG-3′ (reverse primer) |
α-synt (cDNA) |
5′-ATGGCGTCAGGCAGGCGCGC-3′ (forward primer)5′-GCCTCCGTCTGGTCTGCTGC-3′ (reverse primer) |
β1- synt (cDNA) |
5′-CAGTCCCCTTATG AGAAGCTC-3′ (forward primer)5′-TCAGGCCACGCCCAGTC-3′ (reverse primer) |
GAPDH |
5′-ATCACTGCCACCCAGAAGACT-3′ (forward primer)5′-CATGCCAGTGAGCTTCCCGTT-3′ (reverse primer) |
β1-synt (3′UTR) |
5′-ACAGAGGGCGACTTGCCTTTGG-3′ (forward primer)5′-GGAGACAGCATCCAGGGGTGTGG-3′ (reverse primer) |
β1-synt (3′UTR mut1) |
5′-CAGGACTGTGACATCAGGCAGTGCAACATGCACAGCCAGGTTCAGGTT-3′ (forward primer)5′-AACCTGAACCTGGCTGTGCATGTTGCACTGCCTGATGTCACAGTCCTG-3′ (reverse primer) |
β1-synt (3′UTR mut2) |
5′-AGCCAGGTTCAGGTTTAAGGAACATCCCAGCCTCCTCTAT-3′ (forward primer)5′-ATAGAGGAGGCTGGGATGTTCCTTAAACCTGAACCTGGCT-3′ (reverse primer) |
miR-222 |
5′-GAGACCCAGTAGCCAGATGTAGCT-3′
|
U6 |
5′-TTGCGTGTCATCCTTGCGCAGG-3′
|
EGFP |
5′- CAT GGT CCT GCT GGA GTT CGT G -3′ (forward primer)5′- CAG GGG GAG GTG TGG GAG GT -3′ (reverse primer) |