Figure 4.
ERp57 depletion delays oxidative folding of glycosylated substrates. HepG2 cells were depleted of ERp57 or treated with control siRNA and pulse-chase experiments were conducted and analyzed as described in Figure 3. (A) Kinetics of disulfide formation. (B) Secretion kinetics. Black and gray bars represent control and knockdown conditions, respectively. (C) Quantification of oxidative folding. Solid lines, control conditions; dashed lines, knockdown conditions. (D) ER to Golgi transport kinetics. (E) Quantification of the gels in panel D. Error bars represent the average of three independent experiments ± one SD, except in the case of TF which was examined in a single experiment.