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. 2010 Sep 15;21(18):3162–3170. doi: 10.1091/mbc.E09-12-1058

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 2. — Impaired chemotaxis of Ddnhe1⁻ cells is enhanced by DdAip1-Δ382 but suppressed by DdAip1-FL. Chemotactic movement toward a micropipette tip filled with cAMP was recorded for 30 min. (A) Images of Ax2 and Ddnhe1⁻ cells without and with heterologous expression of DdAip1-Δ382. (B) Images of Ax2 and Ddnhe1⁻ cells expressing DdAip1-FL. (C) Morphology and tracking of the indicated cell types determined by drawing and overlapping images of a single cell from frames taken at 1-min intervals. (D) Speed of the indicated cell types, expressed as total distance moved divided by total moving time. The speed of Ddnhe1⁻ cells is significantly decreased by DdAip1-Δ382 but increased by DdAip1-FL. (E) chemotactic index of the indicated cells types, determined as net distance moved divided by total moving distance during the time period. The chemotactic index of Ddnhe1⁻ cells is significantly decreased by DdAip1-Δ382 but increased by DdAip1-FL. Each dot in the scatter plots represents an individual cell in time-lapse images, and data are representative of three independent experiments.