Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 15;21(18):3162–3170. doi: 10.1091/mbc.E09-12-1058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

PMC Copyright notice

Figure 3. — Stimulated F-actin assembly in Ddnhe1⁻ cells is attenuated by DdAip1-Δ382 but rescued by DdAip1-FL. Total F-actin was determined by fluorescence of rhodamine-phalloidin in Triton X-100–insoluble fractions of cells at the indicated times (seconds) after addition of cAMP and expressed relative to F-actin of Ax2 at time 0 in the absence of cAMP. (A) Expression of DdAip1-Δ382 markedly attenuated the first and second peaks of F-actin in Ddnhe1⁻ cells but had no effect in Ax2 cells. (B) Expression of DdAip1-FL in Ddnhe1⁻ cells restores abundance of F-actin in the first peak to that in Ax2 cells.