Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 15;21(18):3162–3170. doi: 10.1091/mbc.E09-12-1058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

PMC Copyright notice

Figure 5. — C-Terminal fragment of Aip1 binds cofilin but not F-actin. (A) Binding of GST-Aip1 full length (FL) and C-terminal fragment (Δ383) and cofilin (20 μM) in the absence of actin was determined by GST-pull-down assay. Cofilin binds both Aip1-FL and -Δ383, but not GST alone. (B) Binding of Aip1 to F-actin in the absence of cofilin was determined by using an actin cosedimentation assay. Polymerized actin (10 μM) was incubated with 0.5 μM Aip1-FL and -Δ383 for 1 h. After centrifugation, the supernatant (S) and the pellet (P) fractions were separated and resolved by SDS-PAGE. Aip1-FL but not -Δ383 binds to actin filaments.