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. 2010 Sep 15;21(18):3193–3204. doi: 10.1091/mbc.E10-04-0324

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 4. — Coimmunoprecipitation analysis of anillin and mDia2. (A and B) Anillin specifically immunoprecipitates mDia2. (A) GFP-mDia2 constructs were expressed in HEK293F cells, and the cell extracts were immunoprecipitated with an anti-anillin antibody and were analyzed by immunoblotting with anti-GFP or anti-anillin antibodies. M1041A: a mutatin in DAD core. (B) GFP-ARR-DD of mDia1, mDia2, or mDia3 construct was expressed in HEK293F cells, and the cell extracts were precipitated with anti-anillin antibodies and analyzed by immunoblotting with anti-GFP or anti-anillin antibodies. (C) mDia2 binds to the N-terminal region of anillin. GFP-anillin constructs were transfected in HEK293F cells, and the cell extracts were incubated with immobilized GST or GST-mDia2 ARR-CC and bound proteins were analyzed by immunoblotting with anti-GFP or anti-GST antibodies.