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. 2010 Sep 15;21(18):3205–3219. doi: 10.1091/mbc.E09-06-0452

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 6. — CDC17 mRNA levels are unchanged in not4Δ mutants. ABy448 (W303-1a), ABy013 (mcm10-1), ABy342 (mcm10-1, ubc4Δ), ABy455 (mcm10-1, UBC4-NES), ABy529 (mcm10-1, not4Δ), ABy363 (mcm10-1, ubc5Δ), ABy359 (mcm10-1, rad6Δ), ABy360 (mcm10-1, ubc13Δ), and ABy361 (mcm10-1, mms2Δ) were grown in rich medium to midlog phase at 25°C and shifted to 37°C for 180 min. Total RNA was extracted from cells taken just before the shift to 37°C and after 180 min at 37°C. Semiquantitative RT-PCR was performed on each sample using 30, 6, and 3 ng total RNA with primers specific for either CDC17 or ACT1.