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. 2010 Sep 15;21(18):3205–3219. doi: 10.1091/mbc.E09-06-0452

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 7. — Cdc17 levels are elevated twofold in the presence of Mcm10 in ubc4Δ, UBC4-NES-3HA, and not4Δ cells. (A) Asynchronous cultures of ABy013 (mcm10-1), ABy342 (mcm10-1, ubc4Δ), ABy455 (mcm10-1, UBC4-NES-3HA), ABy363 (mcm10-1, ubc5Δ), ABy359 (mcm10-1, rad6Δ), ABy360 (mcm10-1, ubc13Δ), ABy361 (mcm10-1, mms2Δ), and ABy529 (mcm10-1, not4Δ) were grown at 25°C to midlog phase. Cdc17–3HA and α-tubulin were analyzed by Western blot. (B) The graph shows the fold change in Cdc17 in each strain relative to mcm10-1 (average of 3 separate experiments, bars represent mean ± SD). (C) Asynchronous cultures of ABy448 (W303-1a), ABy607 (ubc4Δ), ABy597 (not4Δ), and ABy764 (ubc5Δ) were grown at 25°C to midlog phase. Cdc17–3HA and α-tubulin were analyzed by Western blot. The values represent the fold change in Cdc17 levels relative to wild-type.