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. 2010 Sep 15;21(18):3220–3231. doi: 10.1091/mbc.E10-01-0023

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 3. — Distinct roles of eIF2α kinases in response to glucose deprivation. PERK^+/+ and PERK^−/− MEFs (A), PKR^+/+ and PKR^−/− MEFs (B) or GCN2^+/+ and GCN2^−/− MEFs (C) were kept in media containing glucose (Con), media lacking glucose (−Gluc), or media containing glucose supplemented with 50 mM of 2-deoxyglucose (2-DG) for 24 or 48 h. The percentage of cells in sub-G1 phase, which represents cell death, was measured by propidium iodide staining and flow cytometry analysis (see also Supplementary Figures 2–4). Histograms show the average cell death ± SEM calculated from six, five, and seven reproducible experiments for PERK, PKR and GCN2 MEFs, respectively. Two-tailed t test: *p < 0.05, **p < 0.01, ***p < 0.001).