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. 2010 Sep 15;21(18):3247–3257. doi: 10.1091/mbc.E10-01-0025

Figure 1.

Figure 1.

noxROS do not regulate HIF-1α accumulation. (A) Quiescent VSMCs were pretreated with VAS2870 (indicated concentrations) for 20 min and then treated or not with Ang II (100 nM) for 4 h. HIF-1α, phospho-p70S6K, total-p70S6K, and α-tubulin levels were then analyzed by Western blot. (B) VSMCs were transfected with a siRNA against p22phox or a control sequence. Quiescent VSMCs were treated or not with Ang II (100 nM) for 4 h. HIF-1α, p22phox, phospho-p70S6K, total-p70S6K, and α-tubulin levels were then analyzed by Western blot.