Figure 3.

mtROS regulate HIF-1α accumulation. (A) VSMCs were transfected with a siRNA against Rieske Fe-S protein (100 nM) or a control sequence. Cells were then serum-deprived overnight and treated or not with Ang II (100 nM) for 4 h. HIF-1α, Rieske Fe-S protein, and α-tubulin levels were analyzed by Western blot. (B) VSMCs seeded on glass-bottomed cell culture dishes were transfected and treated as in A. Cellular mtROS levels were analyzed by MitoSOX staining as described in Figure 2. Scale bars, 10 μm.