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. 2010 Sep 15;21(18):3247–3257. doi: 10.1091/mbc.E10-01-0025

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 9. — mtROS are essential for Ang II–dependent VSMC migration. VSMCs were transfected with a siRNA against HIF-1 or a control sequence (top) or left untransfected (bottom). Cells were serum-starved 24 h after transfection. Quiescent VSMCs (5 × 10⁵ cells/well) were placed in the upper level of a Boyden chamber and allowed to migrate through a collagen-coated microporous membrane for 4 h toward the lower chamber that contained 100 nM Ang II in DMEM only (top) or in DMEM supplemented or not with stigmatellin (1 μM) or myxothiazol (1 μM; bottom). Cells were then fixed, stained, and counted as described in Materials and Methods. Data expressed are an average ± SEM of three independent experiments performed in triplicate. ***p < 0.001 compared with untreated (−) VSMCs.