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. 2010 Sep 15;21(18):3258–3268. doi: 10.1091/mbc.E10-05-0454

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 6. — Confirmation of the phenotypes in RR-mTOR and RRKD-mTOR transgenic mice. (A) TA muscles from wild-type, RR-mTOR, and RRKD-mTOR mice were collected, and whole muscle lysates were subjected directly to Western blot analysis or subjected to immunoprecipiation (I.P.) for the FLAG-tag followed by Western blot analysis with the indicated antibodies. (B and C) Quantification of total mTOR (B) and the ratio of P-mTOR(2481) to total mTOR (C) in whole muscle lysates. The data are expressed as a percentage of the values obtained in wild-type muscles. *, significantly different from wild type, p ≤ 0.05. (D) TA muscles from wild-type, RR-mTOR and RRKD-mTOR mice were cotransfected with HA-Rheb and GST p70^S6k. Immediately after transfection, mice were subjected to a regime of daily vehicle or 1 mg/kg rapamycin injections. At 48 h after transfection, the muscles were collected, and lysates were subjected to Western blot analysis with the indicated antibodies. The ratio of GST P-p70^S6K(389) to total GST p70^S6K (GST P-p70/Total GST p70) was calculated and expressed as a percentage of the values obtained in wild-type vehicle-treated samples. Values are the mean + SEM, n = 3–5/group. *, significantly different from wild-type vehicle-treated samples, p ≤ 0.05. ND, not detected.