Figure 5.

Effect of Dkk1 on the BMP-2–induced increase in ALP activity in PDZRN3-depleted cells. (A) C2C12 cells infected with adenoviral vectors for PDZRN3 KD-1 or Scramb shRNAs were induced to differentiate into osteoblasts by culture for 3 d in low-serum medium containing BMP-2 (50 ng/ml) and the indicated concentrations of Dkk1. Cell lysates were then prepared and assayed for ALP activity. Data are from a representative experiment. (B) ALP activities of cells treated as in A and exposed to Dkk1 at 200 ng/ml. Data are expressed relative to the corresponding value for cells cultured in the absence of Dkk1 and are means ± SEM from nine independent experiments. ***p < 0.001.