Figure 7.

Effects of PDZRN3 depletion on the cytosolic abundance of β-catenin during BMP-2– or Wnt3a-induced osteoblast differentiation. C2C12 cells infected with adenoviral vectors for PDZRN3 KD-1 or Scramb shRNAs were induced to differentiate into osteoblasts by culture for the indicated times in growth medium supplemented with either murine Wnt3a–containing conditioned medium (A) or BMP-2 (100 ng/ml; B) or in low-serum medium containing BMP-2 (100 ng/ml; C). A cytosolic fraction was then prepared from the cells and subjected to immunoblot analysis with antibodies to β-catenin (top panels). The levels of cytosolic β-catenin at 12 h (A), 24 h (B), or 48 h (C) were determined by densitometry; data are expressed relative to the corresponding value for cells expressing the Scramb shRNA and are means ± SEM from six or seven independent experiments (bottom panels). **p < 0.01, ***p < 0.001 versus the corresponding value for cells expressing the Scramb shRNA.