Table 1.
Primer combinations for detection of single nucleotide polymorphism (SNP) using the Williams 82 soybean genomic sequences (http://www.phytozome.net/soybean) on chromosome Gm06 in the region of the Rpp?(Hyuuga) gene
| STS ID | SNPs/Indela | Upper position on Gm06 | Lower position on Gm06 | Forward primer | Reverse primer | PCR length |
|---|---|---|---|---|---|---|
| Satt460 | 43291288 | 43291438 | ||||
| STS06_43617 | 0/1 | 43617983 | 43618457 | ACC AAG AAC TTT AAC AAG ATT TCG | TGG GAT AGG CAA TAA GAT AGA CCA TA | 475 |
| STS06_43621 | 1/0 | 43621002 | 43621457 | TTA TGG TTA ACT TGG TGG GAG | CAG ATC TTC GAC CCG GA | 456 |
| STS06_43731 | 10/1 | 43731429 | 43731946 | GTA GAA CTT ATG GGC CGT | CAT TGC CAC ATC ATA TAA TCG C | 518 |
| STS06_43831 | 3/1 | 43831609 | 43832161 | GTC GGT AGT AAA GAA AGA TG | TGA AAT ATG TTA TTG GGT AGA C | 553 |
| STS06_43930 | 2/2 | 43930463 | 43931013 | TTT AAA CAG TTT GTA TCA AGT CA | TCC TCA ATA CAG CTC TGG | 551 |
| Satt079 | 43950925 | 43951071 | ||||
| STS06_43958 | 18/1 | 43958861 | 43959374 | GCA TGT ACG TTC TTA TTT GTG G | CTC ATT AGT AAG TAA TCA TGG ATT AGC | 514 |
| STS06_43983 | 3/1 | 43983099 | 43983608 | TTC TGC CAT TTC TTC GG | TGT ACT TAC TGT CCT TTC AC | 510 |
| STS06_44058 | 1/0 | 44058853 | 44059359 | GAC AAG ATC AAA TCG TTG GAG | CAG TTT ATT TAC CTG CTG GTT | 507 |
| Sat_263 | 44188149 | 44188270 | ||||
| STS06_44257 | 26/0 | 44257478 | 44257987 | TCT CAT CTA GGG TGT TGG C | ATT AGC ATC AGA GTT GAA CGA | 510 |
| STS06_44421 | 5/1 | 44421514 | 44422064 | CAT GTA TAG AAG TTG GTT GAG T | GTG CAA CAA TAC CCG GAG | 551 |
| STS06_44422 | 7/0 | 44422369 | 44422877 | TGC CCA TCA ACA GCA TA | TGC ATG ACA CAA AGG TA | 509 |
| STS06_44947 | 1/0 | 44947224 | 44947726 | ATA AGC CTG TTC ATT TAA TTT CTC C | GTC ACT AAC CAA ATC CAG GTC | 503 |
| Staga001 | 45427178 | 45427426 | ||||
| Satt307 | 46286824 | 46286980 |
aIndicates the number of SNPs/indels identified between Dillon versus Hyuuga