Table 2.

List of primers and concentrations used in high-resolution melting analysis with unlabeled probes

Primer name		Primer sequence (5′-3′)	PCR (μM)	SNP Position on Gm06 (Dillon/Hyuuga)
SNP06-43621	F	CTG GGC TGA AGA AAC CAA C	0.2
	R	GCC ATT CTT CTT ACC ATG CT	1.0
	Probe	TGA GAG TGG TAC TAT CAC AGG ACA AAA ATA	1.0	43621233 (T/A)
SNP06-43731	F	GTA GAA CTT ATG GGC CGT	0.2
	R	ATC TTC TCC TTG AAG TGT CAT C	1.0
	Probe	ATC AAT GGC AGC GGA ATG GAG	1.0	43731481 (T/C)
SNP06-43983	F	TTC TGC CAT TTC TTC GG	0.2
	R	GAC TGC CTA GAT TTC AAC CT	1.0
	Probe	TGC AGC CAG TTC ATC AAT CCT CTC	1.0	43983266 (C/T)
SNP06-44058	F	GAC GAT TCA AAT CTC TTC C	1.0
	R	CCA TTC ATA GTG AAC TCA AC	0.2
	Probe	ATC TGG CCC TTT GAC CCT	1.0	44058927 (G/T)
SNP06-44421	F	CCA CTT AAT GAG GTA ACG GT	0.2
	R	TGA CTT CAG TAA TTC AAA TGT TGG G	1.0
	Probe	GGC ACA ACC ACA TCC ATC TCA C	1.0	44421992 (C/T)