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. 2010 Aug 25;2010:107075. doi: 10.1155/2010/107075

Table 1.

Primers used for quantitative RT-PCR. For RT-PCR, we analyzed samples on an MiQ machine using a FastStart SYBR Green Master Mix, with the following primer sets.

Gene Primer set Gene accession number
LAMA3 5′-GGCTCACTCTGTATTGTTGG NM_000227
5′-ACAGAGACTGCTTTGGTGTG
LAMB3 5′-TGATGGACAGGATGAAAGAC NM_000228
5′-GGAAGCTGTAGCATCACTTG
LAMC2 5′-GAAGCCCAGAAGGTTGATAC NM_005562
5′-GTGAGTGTTCTGGAGCAAAG
GAPDH 5′-ATGACATCAAGAAGGTGGTG NM_002046
5′-CTGTAGCCAAATTCGTTGTC
PPIA 5′-CAAATGCTGGACCCAACACA NM_021130
5′-TGCCATCCAACCACTCAGTC