Table 2.
Selected Entries for Putative Nucleolar SUMO2 Substrates from MaxQuant Analysis
| Number in Figure 1 | Protein Names | Gene Names | Uniprot | ELUATE Seq. Cov. (%) | ELUATE Log2 H/L Norm. | ELUATE H/L Sign. A | ELUATE H/L Count | INPUT Whole H/L Sign. B | SUMO Site? | Other Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Alkaline phosphatase; tissue-nonspecific isozyme | ALPL | P05186 | 15.6 | 2.46E | 1.04E-03 | 3 | 6.94E-21 | No | – |
| 2 | H/ACA ribonucleoprotein complex subunit 4 | DKC1 | A8MUT5 | 24.5 | 3.34 | 1.30E-05 | 10 | 0.305 | Yes | Manza et al., 2004; Blomster et al., 2009 |
| 3 | H/ACA ribonucleoprotein complex subunit 2 | NHP2 | Q9NX24 | 39.9 | 5.26 | 1.56E-11 | 3 | 0.177 | Yes | Blomster et al., 2009; Golebiowski et al., 2009 |
| 4 | Nucleolar phosphoprotein p130 | NOLC1 | Q14978 | 8.2 | 2.86 | 1.66E-04 | 7 | 0.292 | Yes | Golebiowski et al., 2009 |
| 5 | Nucleolar protein 58 | NOP58 | Q9Y2X3 | 53.7 | 3.61 | 2.68E-06 | 101 | 3.15E-02 | Yes | Zhao et al., 2004; Manza et al., 2004; Schimmel et al., 2008; Golebiowski et al., 2009 |
| 20 | Cyclin-dependent kinase inhibitor 2A; isoform 4 | CDKN2A | Q8N726 | 39.9 | 5.29 | 1.20E-11 | 6 | 5.67E-18 | No | – |
| 21 | Glucose-6-phosphate isomerase | GPI | P06744 | 45.9 | 2.24 | 2.59E-03 | 36 | 0.497 | Yes | Manza et al., 2004 |
| 22 | KLHDC3 protein | KLHDC3 | Q96GH7 | 22.9 | 2.14 | 3.82E-03 | 5 | – | No | – |
| 23 | Nuclear factor 1 X-type | NFIX | Q14938 | 10.2 | 2.30 | 2.00E-03 | 2 | – | Yes | Golebiowski et al., 2009 |
| 24 | PDZ and LIM domain protein 7 | PDLIM7 | Q9NR12 | 23.9 | 2.24 | 2.54E-03 | 12 | 1.77E-06 | Yes | – |
| 25 | Small ubiquitin-related modifier 3 | SUMO3 | A8MU27 | 17 | 5.70 | 2.93E-13 | 18 | 1.16E-07 | Yes | – |
Same as Table 1 except that the first entry of each column for protein groups with ratio H/L significance A < 0.01 (with ratio count >1) is shown.