Table 2.
Expression vectors.
| Name | Tag description | LIC class | Ab resistance and compatibility | Accession Nos. |
|---|---|---|---|---|
| pNIC28-Bsa4 | N-terminal His6, TEV-cleavable (23 aa) MHHHHHHSSGVDLGTENLYFQ∗SM | 1 | Kanamycin; pET | EF198106 |
| pLIC-SGC1 | N-terminal His6, TEV-cleavable (23 aa) MHHHHHHSSGVDLGTENLYFQ∗SM | 1 | Ampicillin; pET | EF456737 |
| pNIC-CTHF | C-terminal His6-Flag, TEV-cleavable. AENLYFQ∗SHHHHHHDYKDDDDK | 2 | Kanamycin; pET | EF199844 |
| pNIC-CH | C-terminal His6, not cleavable. HHHHHH | 3 | Kanamycin; pET | EF199843 |
| pNIC-NH-TrxT | N-terminal His6, Thioredoxin, TEV-cleavable | 1 | Kanamycin; pET | GU269914 |
| pNIC-Zb | N-terminal His6, Z-basic domain (Hedhammar and Hober, 2007), TEV-cleavable. | 1 | Kanamycin; pET | GU452710 |
| pBEN1-SGC | N-terminal SET (solubility-enhancing) and SBP (streptavidine-binding peptide) tags, TEV-cleavable. | 1 | Kanamycin, pET | GU725055 |
| pFB-LIC-Bse | Baculovirus cloning vector. N-terminal His6, TEV-cleavable (22 aa). MGHHHHHHSSGVDLGTENLYFQ∗SM | 1 | Ampicillin; transposition in the bacterial strain DH10Bac (InVitrogen) to generate recombinant baculovirus DNA | EF199842 |