Figure 2.

Immunolocalization of AGS3 in ARPKD rat kidneys. AGS3 localization is performed in the kidneys of SD (A, C, and D) and PCK (B, E, and F) rats. Affinity-purified AGS3 antibody (pep32) is incubated at a 1:250 dilution on kidney sections from SD (C and D) and PCK (E and F) rats. As a negative control, SD (A) and PCK (B) rat kidneys are incubated with the primary AGS3 antibody in the presence of the competing AGS3 peptide conjugate or normal rodent serum (data not shown). The brown diaminobenzidine staining demonstrates the specific localization of the AGS3 protein within distinct cell types in the kidney. Sections are counterstained with hematoxylin. Arrowhead in E signifies the magnified view of the area in F. n = 4 kidneys per group.