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. 2010 Aug;21(8):1326–1333. doi: 10.1681/ASN.2009091001

Figure 1.

Figure 1.

hi3417 and hi2211 are zygotic loss-of-function mutants of ift57 and ift172, respectively. (A and B) A wild-type sibling and a mutant at 4 dpf in side views. Box with dashed line: cyst (in mutant) and the lack of cyst (in wild-type sibling) enlarged in insets. (C and D) Loss of wild-type ift172 and ift57 transcripts in hi2211 and hi3417 mutants at 33 hpf, respectively. Upper panels: RT-PCR with a pair of primers spanning the proviral insertion site. Lower panels: loading controls with a pair of elf1-specific primers using cDNAs serially diluted 1:1, 1:10, 1:100, and 1:1000. (E) RT-PCR time course for ift172 and ift57 using wild-type samples. (F) Western blot against Ift57 (Ift57). β-Tub is used as loading control. β-Tub, β-Tubulin; ctrl, uninjected embryo control; ift57MO, ift57 morphants; wt, wild type; 4-32c, 4- to 32-cell stage; 30%–27h, mixture of samples from 30% epiboly to 27 hpf; 36h, 36 hpf; 50h, 50 hfp; 5d, 5dpf; 16–64 cell, sample from embryos at the 16- to 64-cell stage; 20s, samples from embryos at the 20-somite stage.