Figure 3.

Inactivation of pk1 leads to kidney cyst formation and disorganization of basal bodies. (A and B) A control embryo and a pk1 morphant raised with 1-phenyl-2-thiourea at 3 dpf in side views. Insets: enlarged areas showing a cyst (B) and the lack of cyst (A). (C and D) Pronephric ducts labeled with anti-Cdh17 in side views. (E through H) Basal bodies as shown by anti–γ-Tub labeling and cilia by anti-a-Tub labeling. Arrows point to arrayed basal bodies, whereas arrowheads point to clusters of disorganized basal bodies. a-Tub, acetylated Tubulin; ctrlMO, embryos injected with a standard control morpholino; γ-Tub, γ-Tubulin; pk1MO, embryos injected with pk1 morpholino.