Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR Oligonucleotide Primers and Probes
| Target | Type | Sequence (5′-3′) |
|---|---|---|
| VZV | F | CGC GTT TTG TAC TCC GGG |
| R | ACG GTT GAT GTC CTC AAC GAG | |
| Probea | TGG GAG ATC CAC CCG GCC AG | |
| HSV-1 | F | TGG TAT TGC CCA ACA CTT TCC |
| R | GCG CCA GGC ACA CAC AT | |
| Probe | CGT GTC GCG TGT GGT | |
| GAPdH | F | CAA GGT CAT CCA TGA CAA CTT TG |
| R | GGC CAT CCA CAG TCT TCT GG | |
| Probe | ACC ACA GTC CAT GCC ATC ACT GCC A |
F, forward primer; R, reverse primer.
Probes contain 5′-FAM and 3′-BHQ labeled (IDT, Coralville, IA).
