Table 3.
MMP-8 does not regulate PMN migration in vitro
| Buffer | fMLP | KC | ZAS | |
|---|---|---|---|---|
| WT PMNG | 25.6 (2.1)H | 70.4 (3.4) | 64.6 (6.9) | 65.5 (5.1) |
| MMP-8−/− PMN | 29.0 (4.7) | 65.4 (5.7) | 72.2 (16.3) | 70.9 (15.9) |
PMNs isolated from the bone marrow of WT and MMP-8−/− mice were resuspended at 4 × 106/ml in RPMI-HEPES containing 1% albumin and incubated for 4 h at 37°C through in Boyden microchemotaxis assay chambers [28] using buffer alone, 10−6M fMLP, 10−7M KC, or 10% zymosan-activated serum [ZAS as a source of C5a [35]] in 6 replicate lower wells for each experimental condition. PMN that migrated through the pores in the membranes were counted in 6–8 high magnification fields for each well on Diff Quik™-stained membranes.
Data are mean ± SEM number of cells migrating (n = 3 separate experiments). There were no significant differences in the migratory responses of WT vs. MMP-8−/− PMNs to buffer or any of the chemoattractants tested.