Figure 4.

The inhibition by [Pt(O,O′-acac)(γ-acac)(DMS)] of MCF-7 cell migration is concentration and time dependent. Differential cell migration rate was examined using wound closure assay. Cells were treated or not with [Pt(O,O′-acac)(γ-acac)(DMS)] at the indicated concentrations and monitored by microscopy at the indicated times. Migration rate (average ± SD) and degree of wound closure were assessed by measuring the distance between wound edges at indicated time intervals in at last eight randomly chosen regions of three different experiments (means ± SD) normalized to 100% wound closure for control cells. *P < 0.05, **P < 0.01, ***P < 0.001; values different from control. Average velocities for the three different experiments are given under photographs.