Figure 8.

[Pt(O,O′-acac)(γ-acac)(DMS)] induces p38MAPK activation through ROS production. (A) MCF-7 cells were treated or not with 0.50 µmol·L⁻¹[Pt(O,O′-acac)(γ-acac)(DMS)] for the indicated time intervals. Cells pre-incubated with apocynin or DPI for 30 min were exposed to [Pt(O,O′-acac)(γ-acac)(DMS)] for 30 min (C) or 1 h (D). ROS production was measured by NBT reduction. Values with shared letters are not significantly different according to Bonferroni/Dunn post hoc tests. (C) Cell fractions (cytosol and membranes for translocation studies) were analysed by Western blotting with specific anti-PKC-ε antibody. The purity of fractions was tested by immunoblotting with anti β-actin and anti-α subunit of Na⁺/K⁺ATPase monoclonal antibodies. (D) Cell lysates were analysed by Western blotting with anti-phosphorylated-Src or anti-total (unphosphorylated and phosphorylated) p38MAPK and phosphorylated-p38MAPK (p-p38MAPK) antibodies. Control loadings are shown by β-actin. The figures are representative of four independent experiments.