Table 2.
Haemodynamics, morphology, β-adrenoceptor density and noradrenaline concentration without and with ROS scavenging by vitamins C and E in sham and HF rabbits
| Sham | Sham-Vit | HF3 | HF3-Vit | |
|---|---|---|---|---|
| Heart rate (bpm) | 223 ± 6 | 220 ± 4 | 259 ± 11A | 241 ± 5 |
| LVEDD (mm) | 16.6 ± 0.2 | 16.7 ± 0.2 | 19.8 ± 0.5A | 18.8 ± 0.2A |
| Fractional shortening (%) | 31.9 ± 1.0 | 31.1 ± 0.7 | 12.9 ± 0.7A | 18.7 ± 0.9AB |
| Noradrenaline (ng·mL−1) | 2.19 ± 0.37 | 1.96 ± 0.26 | 5.82 ± 0.76A | 5.37 ± 1.12A |
| ß-Adrenoceptor density (fmol·mg−1) | 62 ± 5 | 67 ± 4 | 37 ± 3A | 53 ± 7AB |
| Cardiomyocyte cross-sectional area (µm2) | 227 ± 6 | 211 ± 4 | 306 ± 6A | 259 ± 9AB |
| Number of TUNEL-positive cardiomyocytes [/(1000*mm2)] | 4 ± 3 | 19 ± 7 | 64 ± 4A | 18 ± 4A |
| Phospho p38-MAPK/total p38-MAPK (relative to sham) | 1.00 ± 0.30 | 4.79 ± 0.80A | 2.47 ± 0.71A | 3.25 ± 0.55A |
| Phospho HSP27/total HSP27 (relative to sham) | 1.00 ± 0.25 | 1.52 ± 0.55 | 2.70 ± 0.66A | 2.90 ± 0.28A |
Mean ± SEM.
A
p < 0.05 versus Sham.
B
p < 0.05 versus HF3.
HF3, heart failure rabbits, 3 weeks (n = 8); HF3-Vit, heart failure rabbits (3 weeks) receiving vitamins C and E (n = 6); LVEDD, left ventricular end-diastolic diameter; ROS, reactive oxygen species; Sham, sham-operated rabbits (n = 7); Sham-Vit (n = 7), sham-operated rabbits receiving vitamins C and E; TUNEL, TdT-mediated dUTP nick end labelling.