Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 17;49(37):8155–8168. doi: 10.1021/bi100286n

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 American Chemical Society

This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.

PMC Copyright notice

Quantification of microarray hybridization results. RNRspBs was folded in (A) 140Na/80HEPES/10Mg, (B) 135K/25Na/50HEPES/10Mg, and (C) 135K/25Na/50HEPES/1MNa buffers and hybridized to an isoenergetic microarray at 4 °C for 24 h. Probe binding strength is represented by normalized intensity: normalized intensity = (average intensity − background intensity)/(average intensity − background intensity)_{probe 17}, where average intensity is the average of three spots of the same probe. Probes with normalized intensities that are more than one-third of the maximum normalized intensity (red line) are strong binding. Probes with normalized intensities between one-third and one-ninth of the maximum normalized intensity (green line) are intermediate binding. Probes with normalized intensities that are less than one-ninth of the maximum normalized intensity are weak or no binding.