Figure 3.

PGE₂ suppresses the production of endogenous CCL19 in DCs induced to mature by TNFα, IFNα, or TLR ligands; poly-I:C or LPS: stability of the maturation-imprinted ability to produce CCL19. (A) CCL19 mRNA (left) and CCL19 protein expression (right) in αDC1s and sDCs. Representative data from one of 6 different donors. (B) CCL19 secretion by DCs exposed for 48 hours to IFNα or PGE₂ (or both) in absence or presence of maturation factors, TNFα, poly-I:C (TLR3-ligand), or LPS (TLR4 ligand). Cumulative data (mean ± SEM) from 3 different donors. (C) CCL19 levels in αDC1s or sDCs during maturation or after maturation with or without CD40L. Cumulative data (mean ± SEM) from 3 different donors.