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. 2010 May 26;116(9):1460–1468. doi: 10.1182/blood-2009-06-222943

Figure 3.

Figure 3

CAL-101 inhibits the PI3K/Akt and ERK pathway. (A) INA-6 cells were cultured with CAL-101 (0-1.0μM) or LY294002 (0-20μM) for 12 hours. Actin Ab was used as a loading control. (B) INA-6 and MM.1S cells were cultured with CAL-101 (0-5.0μM) for 6 hours. (C) LB and INA-6 cells were cultured with CAL-101 (1.0μM) for 0 to 6 hours. Whole-cell lysates were subjected to immunoblotting using AKT, P-AKT (Ser473 and Thr308), ERK1/2, P-ERK1/2, P-PDK1, and P-FKHRL Abs. α-Tubulin is used as a loading control.