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. 2010 Sep 14;5(9):e12508. doi: 10.1371/journal.pone.0012508

Figure 4. Assessment of coral O2 − detoxifying activity using photochemically generated superoxide spikes.

Figure 4

(A). A composite of two FeLume runs in which 55 nM O2 − spikes were added to filtered seawater (FSW; open symbols) and to seawater previously incubated with a coral (CSW, closed symbols). Superoxide concentrations peaked when the spike reached the detector (∼at 15 sec) and then it decayed back to background values. The antioxidant released by the coral resulted in a faster decay of the O2 − spike as compared with FSW. (B). Same data as in A but plotted in a log-linear plot as the natural logarithm of [O2 −] against time. The slopes of the lines stand for pseudo first-order O2 − decay constants (kCSW or kFSW), which are used to quantify the antioxidant activity. (C). Effect of the coral dimensions (volume or weight) on the antioxidant activity it released (expressed as superoxide decay constant - kCSW). (D). Effect of incubation length on the antioxidant activity released by coral (expressed as superoxide decay constant - kCSW).