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. 2010 Sep 14;5(9):e12723. doi: 10.1371/journal.pone.0012723

Figure 1. PrP species in healthy and scrapie-affected voles.

Figure 1

A: Schematic representation of full length PrP (FL) and PrP fragments (C1 and C2) generated by α and β cleavages. The location of SAF84, 12B2 and SAF32 epitopes used for the FL/C1/C2 discrimination are shown. B and C: Normal brain homogenates (NBH) and scrapie brain homogenates (SBH) of voles were analyzed by western blot using SAF84 (B) and 12B2 (C). The samples, were analyzed either before or after deglycosylation (N-Gly F) as indicated. The brackets on the left indicate the position of glycosylated and unglycosylated bands of FL, C2 and C1: from 35 kDa to 27 kDa for FL, from 26 kDa to 18 kDa for C2 and from 24 kDa to 16 kDa for C1. These PrP species are reduced to single unglycosylated PrP bands after deglycosylation, which are indicated by dashes on the right of the blots. In SBH, PrPSc dimers (Dim) are also indicated. In NBH both C1 and C2 were present, although C2 was poorly represented; in contrast in SBH C2 fragment was the most abundant PrP species while C1 was barely detectable. Tissue equivalents (TE) loaded per lane were 0,15 mg and 0.5 mg for samples respectively before and after PK digestion. Molecular weight markers were loaded into the last lane of each blot. The positions of MW markers are 15, 20, 25, 37 and 50 kDa.