Figure 1. KDM1 and HDAC1 form heteromeric complexes in GC-1 cells.

(A) Endogenous KDM1 and HDAC1 were co-immunoprecipitated with specific antibodies against KDM1 and HDAC1. Total GC-1 protein extract was used as input control. Immunoglobulin G (IgG), Rabbit IgG (RIgG), mouse IgG (MIgG). (B) Western blot analyses of HDAC1 and KDM1 in protein extracts isolated from GC-1 cells grown in culture medium (control, ctr), or in culture medium containing either dimethylsulphoxide (DMSO; TSA solvent), or tranylcypromine (T), or trichostatin A (TSA), or tranylcypromine and TSA (T+TSA). Western blots were reprobed with an antibody against beta-Actin as a loading control. Treatment of GC-1 cells with tranylcypromine and TSA does not alter protein levels of KDM1 and HDAC1.