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. 2010 Sep 14;5(9):e12727. doi: 10.1371/journal.pone.0012727

Figure 8. KDM1- and HDAC1-containing protein complexes are located at the Gfra1 promoter in GC-1 cells.

Figure 8

(A) Schematic representation of the Gfra1 promoter. Numbers depict the positions of primer pairs used for ChIP-qPCR relative to the corresponding transcriptional start site. (B,C) ChIP-qPCRs demonstrate occupancy of the Gfra1 promoter by KDM1- (B) and HDAC1-containing protein complexes (C). ChIP assays were performed on GC-1 cells that have been cultured in the presence of dimethylsulphoxide using either a specific antibody (recognizing either KDM1 (black bars) or HDAC1 (grey bars)) or the corresponding IgG controls (white bars), (rabbit IgG  =  RIgG; mouse IgG  =  MIgG). Each bar represents the ChIP-qPCR results of two independent, pooled experiments. Signals of target DNA received from IgG controls served as base and were defined as one. Fold change represents the 2∧-(ΔΔCT) value, where the normalized IgG signal is subtracted from the normalized signal of the specific antibody. QPCR results were analyzed on an agarose gel and shown underneath the corresponding graph.