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. 2010 Sep 14;5(9):e12743. doi: 10.1371/journal.pone.0012743

Figure 2. Characteristics of the mouse GF-iPS cells.

Figure 2

(A) GF-iPS cell colonies (upper panels: pGF-iPS-4F-1, lower panels: mGF-iPS-3F-4) positively stained for ALP. Scale bar; 30 µm. (B) Bisulfite sequencing of the Nanog and Oct3/4 promoters revealed that CpGs in the parental mGFs were converted to a demethylated state in the GF-iPS cells induced by the three or four factors, resulting in a methylation pattern similar to that of mouse ES cells. The numbers in the panel indicate CpG loci respective to the transcription start site (TSS) of the genes (blue: untranslated region, yellow: translated region). (C and D) RT-PCR analysis of ES cell marker genes (Nanog, ERas, Rex1) and endogenous Oct3/4, Sox2, Klf4 and c-Myc genes in GF-iPS-4F clones (C) or GF-iPS-3F clones (D), mouse ES cells, parental mGFs and SNL feeder cells. GAPDH was used as a loading control.