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. 2010 Sep 14;5(9):e12729. doi: 10.1371/journal.pone.0012729

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) GHSR and NTPDase2 are co-localized in a subset of NTPDase2-positive cells (arrow). (B) GHSR and PLCβ2 are co-localized in a subset of PLCβ2-positive cells (arrow). (C) GHSR and α-gustducin are co-localized in a subset of α-gustducin-positive cells. Arrow, cells expressing both. (D) GHSR and NCAM are co-localized in a subset of NCAM-positive cells. Arrow, cell expressing both. (E) GHSR and PGP9.5 are co-localized in a subset of PGP9.5-positive cells (arrow). (F) GHSR and Shh are co-localized in a subset of Shh-positive cells (arrows). (G) GHSR and PLCβ2 are stained in ghrelin null mice as a negative control. (H) GHSR and PLCβ2 are co-stained after the ghrelin antibody was omitted. Scale bars, 20 µm. Blue is TO-PRO-3 nuclear stain.