Table 4. Potential factors contributing to observation of somewhat different obese phenotype noted with “L-FABP null/GFP overexpressor” mice.
Differences were derived by comparisons of methodology reported for “L-FABP null/GFP overexpressor mice” [143, 163-165, 182, 197] with those for the original “L-FABP null mice” mice [45, 56, 59, 71, 98, 142, 152, 153, 162, 167, 176, 177]. Additional references in the table refer to other publications demonstrating such differences in factors/parameters can affect mouse phenotype/obesity/lipid metabolism.
Factor | Parameter | Affects Mouse Phenotype/Lipid Metabolism/Obesity | Additional References |
---|---|---|---|
Construct | Lipid phenotype | Yes | [198-200] |
Peptide fragments? | Yes | [201-208, 208, 209, 209-215] | |
GFP overexpression | Yes | [216-221] | |
Backcrossing | C57BL/J substrain more obesity susceptible | Yes | [222], http://jaxmice.jax.org/jaxnotes/511/511n.html |
Backcross generation number of L-FABP null | No | [98] | |
Control mice | Vendor supplied vs wild-type littermates | Yes | |
Age | Yes | [223-225] | |
Sex | Yes | [58, 169] | |
Diets | Vendor source, non-defined, not phytol free, not phytoestrogen free | Yes | [58, 168, 171, 172] |
Ad libitum feeding, length of time on high fat diet | Yes | ||
Intestinal microflora | Obesity | Yes | [226-231] |