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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Anat Rec (Hoboken). 2010 Sep;293(9):1455–1469. doi: 10.1002/ar.21193

Figure 6. Loss of Zyx102 expression by RNA interference is pharate adult lethal when using Act5C-GAL4.

Figure 6

A. F1 progeny larvae of Drosophila crosses carrying the Act5C-GAL4 driver and either UAS-zyx102-dsRNA or UAS-lacZ were sorted via genetic markers. Nearly 100% of larvae pupated (data not shown). Fewer than 5% of those carrying Act5C-GAL4 and UAS-zyx102-dsRNA eclosed as adult flies, whereas greater than 90% of those carrying either one of those elements, whether or not UAS-lacZ was present, eclosed as adults. ns=not significant.***p<0.001.

B. Shown are pharate adult flies carrying Act5C-GAL4 and UAS-zyx102-dsRNA. The vast majority of these flies were incapable of escaping from the pupal case.

C. Rare knockdown flies (Act5C-GAL4 and UAS-zyx102-dsRNA) were able to escape the pupal case. Though the legs of these flies could twitch and their external morphology was normal, they did not undergo post-eclosion maturation (the wings did not inflate, the body did not tan or harden, and flies were unable to walk), and soon died. Control flies (w1118; P{UAS-zyx102-dsRNA}16c) at 24 hr post-eclosion are shown for comparison.