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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Anat Rec (Hoboken). 2010 Sep;293(9):1455ā€“1469. doi: 10.1002/ar.21193

Table 1.

Terminal cell labeling

Sample # VNC prepared # expected to carry pAS7 # showing labeled terminal cell processes # showing no labeling # showing background labeling # with isolated cells labeled
Positive controla 7 7 7 0 0 0
Negative controlb 5 0 0 2 3 1d
Test samplesc 10 5 3e 0 4f 3g
a

Positive control: P{UAST-zyx102-dsRNA}16c + pAS7 (1 or 2 copies)

b

Negative control: P{UAST-zyx102-dsRNA}16c

c

Test samples: Pharate adult lethal (Act5C-GAL4 + P{UAST-zyx102-dsRNA}16c) +/āˆ’ 1 copy pAS7

d

One negative sample (from previous column) showed background labeling that resembles an isolated cell (Fig. 8B)

e

Samples likely to be zyx102-dsRNAi + Act5C-GAL4 + pAS7 (Fig. 8Cā€“D)

f

Samples likely to be zyx102-dsRNAi + Act5C-GAL4 (Fig. 8F)

g

Label appears to be expressed in cells that do not have labeled processes (Fig. 8E)