Table 1.
Sample | # VNC prepared | # expected to carry pAS7 | # showing labeled terminal cell processes | # showing no labeling | # showing background labeling | # with isolated cells labeled |
---|---|---|---|---|---|---|
Positive controla | 7 | 7 | 7 | 0 | 0 | 0 |
Negative controlb | 5 | 0 | 0 | 2 | 3 | 1d |
Test samplesc | 10 | 5 | 3e | 0 | 4f | 3g |
Positive control: P{UAST-zyx102-dsRNA}16c + pAS7 (1 or 2 copies)
Negative control: P{UAST-zyx102-dsRNA}16c
Test samples: Pharate adult lethal (Act5C-GAL4 + P{UAST-zyx102-dsRNA}16c) +/ā 1 copy pAS7
One negative sample (from previous column) showed background labeling that resembles an isolated cell (Fig. 8B)
Samples likely to be zyx102-dsRNAi + Act5C-GAL4 + pAS7 (Fig. 8CāD)
Samples likely to be zyx102-dsRNAi + Act5C-GAL4 (Fig. 8F)
Label appears to be expressed in cells that do not have labeled processes (Fig. 8E)