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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cell Signal. 2010 Jul 24;22(12):1849–1857. doi: 10.1016/j.cellsig.2010.07.012

Figure 3. VEGF synthesis in hyperglycemic kidneys.

Figure 3

A. VEGF expression at the protein level was measured on kidney cortex homogenates by immunoblot using a monoclonal antibody directed against VEGF. Actin was used as a loading control. The lower panel shows combined data obtained on kidneys from 5 individual mice for each experimental condition. B. VEGF expression at the mRNA level was measured by RT-qPCR as described in the Methods section. Data was obtained on RNA extracted from kidneys from 5 individual mice for each experimental condition. **p<0.01, ns = not significant by ANOVA.