Fig. 1.
Inhibition of NMDA/glycine-induced ion flow through recombinant NMDAR channels by con-G, con-G[Q6A], con-G[1–11]. Peptides (40 μM) were applied as indicated by the lower horizontal line accompanying each trace, while NMDA (100 μM)/glycine (10 μM) was applied throughout each experiment (as denoted by the upper line). The recombinant NMDARs consisted of the subunit combinations, NR1a/NR2A, NR1a/NR2B, NR1b/NR2A, and NR1b/NR2B. (A–D) con-G; (E–H) con-G[1–11]; (I–L) con-G[Q6A]. (M) Comparison of maximal inhibition by con-G, con-G[Q6A], and con-G[1–11] of steady state whole cell currents in HEK-293 cells transfected with the four different NMDAR subunit complexes. Voltage-clamped recordings were obtained at −70 mV, pH 7.35, 25o C.